bg) mice, but they engraft poorly with human immune systems. In contrast, human skin graft integrity is excellent on CB17- scid bg ( SCID. It is suggested that multiple immunological dysfunctions, including cytokine production capability, in addition to functional incompetence of T, B, and NK cells, may lead to the high engraftment levels of xenograft in NOD/SCID/gamma(c)(null) mice. The NOG mouse shows markedly better engraftment of human cells and human tissues than the NOD/scid mouse and also makes possible. Nonobese diabetic (NOD)- scid interleukin-2 gamma chain receptor ( NSG) readily engraft with human immune systems, but human skin graft integrity is poor. The interferon-gamma production from dendritic cells from the NOD/SCID/gamma(c)(null) mouse spleen was significantly suppressed in comparison with findings in 2 other strains of mice. To elucidate the mechanisms involved in the superior engraftment rate in NOD/SCID/gamma(c)(null) mice, cytokine production of spleen cells stimulated with Listeria monocytogenes antigens was compared among these 3 strains of mice. NOD-scid mice: NOD mice combined with Prkdc gene knockout results in a loss of functional T cells, B cells and the complement system, in addition to reducing. These results suggest that NOD/SCID/gamma(c)(null) mice were superior animal recipients for xenotransplantation and were especially valuable for human stem cell assay. Further, even 1 x 10(2) CD34+ cells could grow and differentiate in this strain. That model is even tighter, and affects the innate immune system as well. There's also a NOD/SCID/gamma mouse, which is a NOD/SCID mouse with the IL-2 common gamma chain receptor knockout. Also, impaired ability to make NK cells, which SCID mice make just fine. In addition to the high engraftment rate, multilineage cell differentiation was also observed. When they crossed SCID mice onto the NOD background, they got less leakiness. However, the ability to perform long-term lymphohematopoietic repopulation studies in the NOD- scid stock has been limited by the fact that most of these mice develop lethal thymic lymphomas. In contrast, human skin graft integrity is excellent on CB17- scid bg (SCID.bg) mice, but they engraft poorly with human immune systems. Moreover, NOD-scid mice support higher levels of human lymphohematopoietic cell growth than the C.B-17-scid strain in which the mutation originated. The same high engraftment rate of human mature cells was observed in ascites when peripheral blood mononuclear cells were intraperitoneally transferred. Nonobese diabetic (NOD)- scid interleukin-2 gamma chain receptor ( NSG) readily engraft with human immune systems, but human skin graft integrity is poor. When human CD34+ cells from umbilical cord blood were transplanted into this strain, the engraftment rate in the peripheral circulation, spleen, and bone marrow were significantly higher than that in NOD/Shi-scid mice treated with anti-asialo GM1 antibody or in the beta2-microglobulin-deficient NOD/LtSz-scid (NOD/SCID/beta2m(null)) mice, which were as completely defective in NK cell activity as NOD/SCID/gamma(c)(null) mice. To establish a more appropriate animal recipient for xenotransplantation, NOD/SCID/gamma(c)(null) mice double homozygous for the severe combined immunodeficiency (SCID) mutation and interleukin-2Rgamma (IL-2Rgamma) allelic mutation (gamma(c)(null)) were generated by 8 backcross matings of C57BL/6J-gamma(c)(null) mice and NOD/Shi-scid mice.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |